Daphnia magna ecotoxicogenomics provides mechanistic insights into metal toxicity.

نویسندگان

  • Helen C Poynton
  • Julia R Varshavsky
  • Bonnie Chang
  • Giorgio Cavigiolio
  • Sarah Chan
  • Patricia S Holman
  • Alexandre V Loguinov
  • Darren J Bauer
  • Kelly Komachi
  • Elizabeth C Theil
  • Edward J Perkins
  • Owen Hughes
  • Chris D Vulpe
چکیده

Toxicogenomics has provided innovative approaches to chemical screening, risk assessment, and predictive toxicology. If applied to ecotoxicology, genomics tools could greatly enhance the ability to understand the modes of toxicity in environmentally relevant organisms. Daphnia magna, a small aquatic crustacean, is considered a "keystone" species in ecological food webs and is an indicator species for toxicant exposure. Our objective was to demonstrate the potential utility of gene expression profiling in ecotoxicology by identifying novel biomarkers and uncovering potential modes of action in D. magna. Using a custom D. magna cDNA microarray, we identified distinct expression profiles in response to sublethal copper, cadmium, and zinc exposures and discovered specific biomarkers of exposure including two probable metallothioneins, and a ferritin mRNA with a functional IRE. The gene expression patterns support known mechanisms of metal toxicity and reveal novel modes of action including zinc inhibition of chitinase activity. By integrating gene expression profiling into an environmentally important organism, this study provides experimental support for the utility of ecotoxicogenomics.

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عنوان ژورنال:
  • Environmental science & technology

دوره 41 3  شماره 

صفحات  -

تاریخ انتشار 2007